黄笛

  黄笛，副研究员，硕士生导师。研究方向：功能寡糖和糖蛋白合成生物学。

2008年毕业于大连理工大学，获学士学位，2010年获天津大学硕士学位，2013年获天津大学博士学位。2013年7月进入南开大学工作，现任南开大学泰达生物技术研究院副研究员；主要研究领域为重要功能分子产物（母乳寡糖、代糖、糖蛋白）的生物合成与合成生物学研究，以创造更卓越“大健康”产品、突破“卡脖子”技术为目标，实现重要功能分子的合成生物学智造，进而提升相关产业的技术水平与国际竞争力。定向设计目标糖与药物合成途径，系统解析代谢网络，通过合成生物学策略，定向提高目标有效组分的产量和质量，从而直接服务于工业化生产。重点开发系统生物学、合成生物学技术与功能基因组技术在糖化学中的理论基础及应用，汇聚多学科研究手段，探究解析微生物的生理特性和调控机制，辅以计算机设计开发创建新型原核生物细胞工厂，实现工业化菌株的个性化定制。

以第一或通讯作者在Nature Microbiology、Green Chemistry、Carbohydrate Polymers、Metabolic Engineering、Gut Microbes、Biotechnology Advances、Trends in Biotechnology、FEMS Microbiology Reviews等国际权威期刊发表SCI论文40余篇，获得授权发明专利10余项。

主持国家自然科学基金、国家重点研发项目子课题、天津市自然科学基金项目、广东省自然科学基金项目、深圳市自然科学基金项目、中央高校基本科研项目等若干项。2017年入选天津市“131”第三层次人才。

联系电话：022-65333286

Email：huangdi@nankai.edu.cn

部分代表作

第一和通讯作者（含共同）

1. Li L#, Liu Y#, Liu D#, Wang J#, Wang M#, Xiang B, Qin J, Yao T, Li W, Wu P, Wang Q, Zhang J, Xu Y, Liu M, Wang Y, Ma G, Liu R, Li X, Huai Z, Huang Y, Guo H, Yang B, Feng L, Huang D*, Zhang K*, Wang L*, Liu B*. Microbiota-derived succinate promotes enterohaemorrhagic Escherichia coli virulence via lysine succinylation. Nat Microbiol. 2025; 10(3):749-764.

2. Ma G#, Jiang X#, Yang B#, Li L#, Liu R, Meng Q, Li J, Xie L, Guo H, Liu S, Wang Y, Wang Y, Zhao X, Li Z, Wang Y, Xia M, Huang D*. Development of a High-Efficiency N-acetylneuraminic acid Production Platform through Multi-Pathway Synergistic Engineering. Trends Biotechnol. In Press.

3. Li Z, Wang Y, Zhao X, Meng Q, Ma G, Xie L, Jiang X*, Liu Y*, Huang D*. Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions. Biotechnol Adv. 2025; 79:108514.

4. Wang Q#, Liu R#, Niu Y#, Wang Y, Qin J, Huang Y, Qian J, Zheng X, Wang M, Huang D*, Liu Y*. Regulatory mechanisms of two-component systems in Vibrio cholerae: Enhancing pathogenicity and environmental adaptation. Microbiol Res. 2025; 298:128198.

5. Sun H#, Huang D#, Pang Y#, Chen J#, Kang C#, Zhao M, Yang B*. Key roles of two-component systems in intestinal signal sensing and virulence regulation in enterohemorrhagic Escherichia coli. FEMS Microbiol Rev. 2024; 48(6):fuae028.

6. Wang Y, Tan H, Wang Y, Qin JL, Zhao X, Di Y, Xie L, Wang Y, Zhao X, Li Z, Ma G, Jiang L, Liu B*, Huang D*. High-level biosynthesis of chlorogenic acid from mixed carbon sources of xylose and glucose through a rationally refactored pathway network. J Agric Food Chem. 2024; 72(7):3633-3643.

7. Wang Y, Perepelov AV, Senchenkova SN, Lu G, Wang X, Ma G, Yang Q, Yuan J, Wang Y, Xie L, Jiang X, Qin J, Liu D, Liu M, Huang D*, Liu B*. Glycoengineering directs de novo biomanufacturing of UPEC O21 O-antigen polysaccharide based glycoprotein. Int J Biol Macromol. 2023; 253(Pt 4):126993.

8. Wang Y, Wang X, Ma G, Xie L, Liu D, Wang Y, Zhao X, Su Y, Perepelov AV, Ding P, Zhang X, Xu B, Liu B*, Huang D*. Sustainable production of a polysaccharide-based glycoprotein by simultaneous conversion of glucose and glycerol in engineered Escherichia coli. Green Chem. 2023; 25(12):4818-4832.

9. Jiang X#, Bai J#, Zhang H, Yuan J, Lu G, Wang Y, Jiang L, Liu B, Huang D*, Feng L*. Development of an O-polysaccharide based recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1. Carbohydr Polym. 2022; 277:118796.

10. Wu J#, Liu Y#, Li W, Li F, Liu R, Sun H, Qin J, Feng X, Huang D*, Liu B*. MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization. Gut Microbes. 2022; 14(1):2143216.

11. Yuan J#, Jiang X#, Zhang H, Bai J, Zhang J, Wang Y, Lu G, Xu H, Liu H*, Liu B*, Huang D*. Construction and optimization of a microbial platform for sustainable biosynthesis of poly-N-acetyllactosamine glycoprotein in the cytoplasm for detecting tumor biomarker galectin-3. Green Chem. 2021; 23(7):2668-2684.

12. Jiang X, Bai J, Yuan J, Zhang H, Lu G, Wang Y, Jiang L, Liu B, Wang L, Huang D*, Feng L*. High efficiency biosynthesis of O-polysaccharide-based vaccines against extraintestinal pathogenic Escherichia coli. Carbohydr Polym. 2021; 255:117475.

13. Wang L, Zhu W, Lu G, Wu P, Wei Y, Su Y, Jia T, Li L, Guo X, Huang M, Yang Q, Huang D*, Liu B*. In silico species identification and serotyping for Cronobacter isolates by use of whole-genome sequencing data. Int J Food Microbiol. 2021; 358:109405.

14. Huang D#, Wang Y#, Zhang J#, Xu H, Bai J, Zhang H, Jiang X, Yuan J, Lu G, Jiang L, Liao X, Liu B*, Liu H*. Integrative metabolomic and transcriptomic analyses uncover metabolic alterations and pigment diversity in Monascus in response to different nitrogen sources. mSystems. 2021; 6(5):e0080721.

15. Liu H, Zhang J, Lu G, Wang F, Shu L, Xu H, Li Z, Wang Y, Guo Q, Wu S, Jiang L, Wang C, Huang D*, Liu B*. Comparative metabolomics analysis reveals the metabolic regulation mechanism of yellow pigment overproduction by Monascus using ammonium chloride as a nitrogen source. Appl Microbiol Biotechnol. 2021; 105(16-17):6369-6379.

16. Liu H, Zhang J, Yuan J, Jiang X, Jiang L, Li Z, Yin Z, Du Y, Zhao G, Liu B*, Huang D*. Gene coexpression network analysis reveals a novel metabolic mechanism of Clostridium acetobutylicum responding to phenolic inhibitors from lignocellulosic hydrolysates. Biotechnol Biofuels. 2020; 13:163.

17. Hu D#, Yin Z#, Yuan C#, Yang P, Qian C, Wei Y, Zhang S, Wang Y, Yuan J, Wang M, Reeves PR, Tu L, Chen M*, Huang D*, Liu B*. Changing molecular epidemiology of Vibrio cholerae outbreaks in Shanghai, China. mSystems. 2019; 4(6):e00561-19.

18. Yin Z#, Yuan C#, Du Y#, Yang P, Qian C, Wei Y, Zhang S, Huang D*, Liu B*. Comparative genomic analysis of the Hafnia genus reveals an explicit evolutionary relationship between the species alvei and paralvei and provides insights into pathogenicity. BMC Genomics. 2019; 20(1):768.

19. Liu H, Zhang J, Yuan J, Jiang X, Jiang L, Zhao G, Huang D*, Liu B*. Omics-based analyses revealed metabolic responses of Clostridium acetobutylicum to lignocellulose-derived inhibitors furfural, formic acid and phenol stress for butanol fermentation. Biotechnol Biofuels. 2019; 12:101.

20. Sun L#, Huang D#, Zhu L#, Zhang B, Peng C, Ma T, Deng X, Wu J, Wang W*. Novel thermostable enzymes from Geobacillus thermoglucosidasius W-2 for high-efficient nitroalkane removal under aerobic and anaerobic conditions. Bioresour Technol. 2019; 278:73-81.

21. Wang C*, Wang J, Yuan J, Jiang L, Jiang X, Yang B, Zhao G, Liu B, Huang D*. Generation of Streptomyces hygroscopicus cell factories with enhanced ascomycin production by combined elicitation and pathway-engineering strategies. Biotechnol Bioeng. 2019; 116(12):3382-3395.

22. Wang C#, Huang D#, Liang S. Identification and metabolomic analysis of chemical elicitors for tacrolimus accumulation in Streptomyces tsukubaensis. Appl Microbiol Biotechnol. 2018; 102(17):7541-7553.

23. Huang D, Yang K, Liu J, Xu Y, Wang Y, Wang R, Liu B*, Feng L*. Metabolic engineering of Escherichia coli for the production of 2'-fucosyllactose and 3-fucosyllactose through modular pathway enhancement. Metab Eng. 2017; 41:23-38.

24. Liu H#, Huang D#, Jin L#, Wang C, Liang S, Wen J*. Integrating multi-omics analyses of Nonomuraea dietziae to reveal the role of soybean oil in [(4'-OH)MeLeu]4-CsA overproduction. Microb Cell Fact. 2017; 16(1):120.

25. Huang D*, Liu J, Qi Y, Yang K, Xu Y, Feng L*. Synergistic hydrolysis of xylan using novel xylanases, β-xylosidases, and an α-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017; 101(15):6023-6037.

26. Wang J#, Liu H#, Huang D#, Jin L, Wang C, Wen J. Comparative proteomic and metabolomic analysis of Streptomyces tsukubaensis reveals the metabolic mechanism of FK506 overproduction by feeding soybean oil. Appl Microbiol Biotechnol. 2017; 101(6):2447-2465.

27. Liu H#, Huang D#, Wen J*. Integrated intracellular metabolic profiling and pathway analysis approaches reveal complex metabolic regulation by Clostridium acetobutylicum. Microb Cell Fact. 2016; 15:36.

28. Huang D*, Wang R, Du W, Wang G, Xia M. Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol. Bioresour Technol. 2015; 196:263-272.

29. Wang G#, Huang D#, Li Y, Wen J*, Jia X. A metabolic-based approach to improve xylose utilization for fumaric acid production from acid pretreated wheat bran by Rhizopus oryzae. Bioresour Technol. 2015; 180:119-127.

30. Huang D, Li S, Xia M, Wen J*, Jia X. Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement. Microb Cell Fact. 2013; 12:52.

31. Xia M#, Huang D#, Li S, Wen J*, Jia X, Chen Y. Enhanced FK506 production in Streptomyces tsukubaensis by rational feeding strategies based on comparative metabolic profiling analysis. Biotechnol Bioeng. 2013; 110(10):2717-2730.

32. Wang G#, Huang D#, Qi H, Wen J*, Jia X, Chen Y. Rational medium optimization based on comparative metabolic profiling analysis to improve fumaric acid production. Bioresour Technol. 2013; 137:1-8.

33. Huang D, Wen J*, Wang G, Yu G, Jia X, Chen Y. In silico aided metabolic engineering of Streptomyces roseosporus for daptomycin yield improvement. Appl Microbiol Biotechnol. 2012; 94(3):637-649.